FIGURE

Fig. 4

ID

ZDB-FIG-220212-4

Publication

Höijer et al., 2022 - CRISPR-Cas9 induces large structural variants at on-target and off-target sites in vivo that segregate across generations

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Fig. 4

Schematic view of sequencing reads and editing events at the on-target sites for sh2b3 and ywhaqa, produced by the SIQ software. In each plot, the y-axis displays the fraction of different editing events, and the x-axis their genomic positions. The zero coordinate indicates the Cas9-cleavage site for sh2b3 and ywhaqa. Control samples from uninjected zebrafish displaying the sh2b3 (a) and ywhaqa (b) targeted sites. Only wild-type alleles and sequences containing SNVs are detected in these controls. SIQ results from three individual F0 founder fish edited for sh2b3 (c) or ywhaqa (d). In the CRISPR-Cas9 edited F0 individuals, a large number of distinct insertions, deletions, as well as combinations of insertions and deletions are reported. This indicates a high degree of somatic mosaicism of CRISPR-Cas9 editing events in the F0 individuals. Source data are provided as a Source Data file.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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