FIGURE

Fig. 2

ID

ZDB-FIG-220212-2

Publication

Höijer et al., 2022 - CRISPR-Cas9 induces large structural variants at on-target and off-target sites in vivo that segregate across generations

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Fig. 2

Genome editing was performed in fertilized eggs by microinjection of ribonucleoprotein (RNP) at the single-cell stage. The genome editing experiment results in mosaic heterozygous mutants, mosaic homozygous mutants, or unaffected homozygotes (together referred to as founders). A number of F0 embryos were not injected and used as controls. F1 generation zebrafish were generated by in-crossing randomly selected pairs of adult founders. The offspring of these crossings have stable genotypes with 0 (−/−), 1 (+/−) or 2 (+/+) mutated alleles. Samples were collected for analysis at different stages of the experiment as described in the gray box.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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