PUBLICATION

CRISPR-Cas9 induces large structural variants at on-target and off-target sites in vivo that segregate across generations

Authors

Höijer, I., Emmanouilidou, A., Östlund, R., van Schendel, R., Bozorgpana, S., Tijsterman, M., Feuk, L., Gyllensten, U., den Hoed, M., Ameur, A.

ID

ZDB-PUB-220204-2

Date

2022

Source

Nature communications 13: 627 (Journal)

Registered Authors

Keywords

none

MeSH Terms

Germ Cells
Mutation
RNA, Guide, Kinetoplastida/genetics
Genetic Therapy
Humans
Zebrafish/genetics*
Animals
CRISPR-Cas Systems*
Gene Editing/methods*
DNA

PubMed

35110541 Full text @ Nat. Commun.

Abstract

CRISPR-Cas9 genome editing has potential to cure diseases without current treatments, but therapies must be safe. Here we show that CRISPR-Cas9 editing can introduce unintended mutations in vivo, which are passed on to the next generation. By editing fertilized zebrafish eggs using four guide RNAs selected for off-target activity in vitro, followed by long-read sequencing of DNA from >1100 larvae, juvenile and adult fish across two generations, we find that structural variants (SVs), i.e., insertions and deletions ≥50 bp, represent 6% of editing outcomes in founder larvae. These SVs occur both at on-target and off-target sites. Our results also illustrate that adult founder zebrafish are mosaic in their germ cells, and that 26% of their offspring carries an off-target mutation and 9% an SV. Hence, pre-testing for off-target activity and SVs using patient material is advisable in clinical applications, to reduce the risk of unanticipated effects with potentially large implications.

Genes / Markers

Figures