FIGURE

Fig. 7

ID

ZDB-FIG-211216-260

Publication

Hseu et al., 2021 - The in vitro and in vivo depigmenting activity of pterostilbene through induction of autophagy in melanocytes and inhibition of UVA-irradiated α-MSH in keratinocytes via Nrf2-mediated antioxidant pathways

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Fig. 7

Silencing of Nrf2 suppressed Pt-mediated protective effects in HaCaT cells. (A) Cells transfected with specific siRNA for Nrf2 or nonsilencing control were tested with Pt (5 μM) for the indicated time. The Western blot method measured the expression of total Nrf2 (0.5 h), HO-1 (6 h), NQO-1 (8 h), and γ-GCLC (8 h) proteins. (B) Transfected cells were first treated with Pt (5 μM, 24 h) and then exposed or non-exposed to 3 J/cm2 UVA. DCF fluorescence technique was used to measure the ROS levels. (C) Transfected cells were first treated with Pt (30 μM, 24 h) followed by exposure or non-exposed to 3 J/cm2 UVA to determine α-MSH protein levels through the Western blot method. Results were denoted as mean ± SD of three or more independent experiments. Statistical significance was considered as *p < 0.05, ***p < 0.001 compared to UVA-irradiated transfected cells.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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