ZFIN Image: Hseu et al., 2021, Fig. 7

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Figure Caption

Fig. 7 Silencing of Nrf2 suppressed Pt-mediated protective effects in HaCaT cells. (A) Cells transfected with specific siRNA for Nrf2 or nonsilencing control were tested with Pt (5 μM) for the indicated time. The Western blot method measured the expression of total Nrf2 (0.5 h), HO-1 (6 h), NQO-1 (8 h), and γ-GCLC (8 h) proteins. (B) Transfected cells were first treated with Pt (5 μM, 24 h) and then exposed or non-exposed to 3 J/cm2 UVA. DCF fluorescence technique was used to measure the ROS levels. (C) Transfected cells were first treated with Pt (30 μM, 24 h) followed by exposure or non-exposed to 3 J/cm2 UVA to determine α-MSH protein levels through the Western blot method. Results were denoted as mean ± SD of three or more independent experiments. Statistical significance was considered as *p < 0.05, ***p < 0.001 compared to UVA-irradiated transfected cells.

Acknowledgments

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