FIGURE

Fig. 11

ID

ZDB-FIG-211216-264

Publication

Hseu et al., 2021 - The in vitro and in vivo depigmenting activity of pterostilbene through induction of autophagy in melanocytes and inhibition of UVA-irradiated α-MSH in keratinocytes via Nrf2-mediated antioxidant pathways

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Fig. 11

LC3 knockdown diminished Pt-mediated anti-melanogenic proteins expression in B16F10 cells. Transfection experiment was conducted as described in the methodology. The LC3 expression was silenced using a specific siRNA against LC3. Non-silenced cells functioned as control. (A–E) Transfected cells were treated with 30 μM Pt for the indicated time points to allow the expression of LC3 (24 h) (B), tyrosinase (24 h) (C), TRP-1 (24 h) (D), and TRP-2 (24 h) (E) proteins in both non-silenced (control siRNA) and siLC3 cells. After incubation, cells were harvested, and cell lysate was subjected to the Western blot technique. The β-actin protein functioned as a loading control. Results were denoted as mean ± SD of three or more independent experiments. Statistical significance was considered as *p < 0.05 ***p < 0.001 compared to control siRNA cells and #p < 0.05, ###p < 0.001 compared to Pt-treated control siRNA cells.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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