Fig. 2
- ID
- ZDB-FIG-211216-255
- Publication
- Hseu et al., 2021 - The in vitro and in vivo depigmenting activity of pterostilbene through induction of autophagy in melanocytes and inhibition of UVA-irradiated α-MSH in keratinocytes via Nrf2-mediated antioxidant pathways
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Pt suppressed UVA-induced ?-MSH expression in HaCaT cells and other melanogenic proteins in B16F10 cells. (A) HaCaT cells were first treated with Pt (0?5 ?M) or Rv (10 ?M) for 24 h and then irradiated or not with 3 J/cm2 UVA. After 8 h, cells were collected, proteins were extracted. The Western blot method to determine POMC and ?-MSH protein levels. (B?D) The conditioned medium obtained from Pt pretreated (2.5 or 5 ?M, 24 h) and 3 J/cm2 UVA-irradiated HaCaT cells were tested on B16F10 cells for the indicated time points. The Western blot method determined the expression of CREB, p-CREB (2 h) (B), MITF (4 h) (C) or tyrosinase (24 h) proteins (D). ?-actin or histone proteins were used as loading control proteins. AlphaEaseFC? software was used to measure the immunoreactive protein bands (Genetic Technologies, Inc. Florida, USA). (E) Pt inhibited melanin formation in ?-MSH-stimulated B16F10 cells ? different concentrations of Pt (0?30 ?M, 24 h) were first treated to B16F10 cells, and then stimulated or not with ?-MSH (1 ?M, 24 h). Melanin was quantified as described in the methodology. Results were denoted as mean ± SD of three or more independent experiments. Statistical significance was considered as ***p < 0.001 compared to untreated control and ###p < 0.001 compared to ?-MSH-stimulated cells. |