FIGURE

Figure 8

ID

ZDB-FIG-211201-179

Publication

Chen et al., 2021 - Knockout of mafba Causes Inner-Ear Developmental Defects in Zebrafish via the Impairment of Proliferation and Differentiation of Ionocyte Progenitor Cells

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Figure 8

Analysis of the accumulation of DNA damage in mafba^−/− mutants’ inner-ear. (A) Whole-mount immunofluorescence analysis using the anti-γH2AX antibody in siblings and mafba^−/− inner-ear at 3, 4, and 5 dpf. The white, dotted circle represents the inner ear area. Scale bars: 20 µm. (B) Statistical analysis of the γH2AX-positive cells is shown in (A). (C) The alkaline comet assay showed increased DNA damage in mafba^−/− embryos at 4 and 5 dpf. White arrows show DNA-damaged cells with single- or double-strand breaks. Scale bars: 10 µm. (D) Quantitative analysis of 88 cells from 10 embryos in siblings and mafba^−/− group are shown. (E) Western blot analysis of γH2AX, P-ATM, P-ATR, and p53 in siblings and mafba^−/− zebrafish at 4 dpf. GAPDH was used as the normalized protein control. Data are represented as mean ± SD; *, p < 0.05; ***, p < 0.001.

Expression Data

Antibodies:	Ab2-atr Ab4-atm Ab4-h2afx
Fish:	mafba^hzu17/hzu17
Anatomical Terms:	head inner ear ionocyte
Stage Range:	Protruding-mouth to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mafba^hzu17/hzu17
Observed In:	head inner ear ionocyte ionocyte apoptotic process
Stage Range:	Protruding-mouth to Day 5

Phenotype Detail

Acknowledgments

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