FIGURE

Fig. 5

ID

ZDB-FIG-211122-18

Publication

Bell et al., 2021 - Optogenetic control of receptors reveals distinct roles for actin- and Cdc42-dependent negative signals in chemotactic signal processing

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Fig. 5

Cdc42 is activated downstream of G<sub>i</sub>α family G proteins.

a Schematic indicating the potential for Cdc42 response regulation independent of heterotrimeric G protein signals. b, c Single-pulse stimulations of populations of PLB-985 cells expressing parapinopsina and the Cdc42-TomKat sensor either without (b) or after treatment with 600 ng/ml Pertussis toxin (PTX) to inhibit G_iα-dependent signals (c). b Data are presented as mean ± s.e.m of n_{well replicates} = 16 for all conditions. c Data are presented as mean ± s.e.m (n_{well replicates} = 27 for relative light intensity = 0, n_{well replicates} = 25 for relative light intensity = 5, n_{well replicates} = 26 for relative light intensity = 10, n_{well replicates} = 16 for relative light intensity = 20, and n_{well replicates} = 16 for relative light intensity = 50). Stimulus duration is indicated by the gray bar. Source data are provided as a Source Data file.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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