Expression of bmp genes in the adult zebrafish telencephalon. (A–E) ISH with probes directed against bmp2a (A), bmp2b (B), bmp4 (C), bmp7a (D) and bmp7b (E) mRNA on transverse telencephalic sections. While bmp2a (A), bmp7a (D) and bmp7b (E) appear to be expressed in all telencephalic nuclei, bmp2b (B) and bmp4 (C) expression is more confined to the medial region. Red rectangles in (A–E) indicate the coordinates of the region presented in (F–J), respectively. (F–J) Expression of bmp genes revealed by fluorescent FISH (red) on cross-sections of WT telencephala, together with double immunofluorescent staining for NSCs (glutamine synthetase [GS], green) and post-mitotic neurons (Hu, blue). The bmp genes are co-expressed with Hu in neurons (yellow arrows in F–J) but are not expressed in GS+ NSCs (white arrows). (K–L’’’) BMP2b antibody staining (red) along with antibodies against GS (K–K’’’, green) or NeuroD1 (L–L’’’, blue) shows that Bmp2b is expressed in NeuroD1+ neurons. White rectangles (K,L) represent the region magnified in K’–K’’’ and L’–L’’’, respectively. White arrowheads show the GS+ NSCs (K’–K’’’), yellow arrowheads indicate neurons (L’–L’’’). Scale bar = 20 μm (F–J, K’–K’’’,L’–L’’’), 100 μm (A–E,K,L). Dc: central zone of the dorsal telencephalic area; Dl: lateral zone of the dorsal telencephalic area; Dm: medial zone of the dorsal telencephalic area; Vc: central nucleus of the ventral telencephalic area; Vd: dorsal nucleus of the ventral telencephalic nucleus; Vv: ventral nucleus of the ventral telencephalic area.
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