FIGURE

Fig. 1

ID

ZDB-FIG-211025-179

Publication

Ribera et al., 2021 - The loss of DHX15 impairs endothelial energy metabolism, lymphatic drainage and tumor metastasis in mice

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Fig. 1

Characterization of the Akt and DHX15 signaling crosstalk in endothelial cells.

a The expression of DHX15, Akt1, and Akt2 proteins was evaluated by western blot using cell lysates from wild-type and siL-DHX15-LECs. β-actin was used as a loading control. The densitometric analysis of the protein expression is shown on the bar graph. **p < 0.01 vs. wild-type LEC (n = 3 biologically independent samples for each condition). b The activity of Akt was determined in non-silenced and DHX15-silenced endothelial cells in a kinase reaction using recombinant GSK-3 as substrate. The levels of GSK-3α/β phosphorylation were analyzed by western blot using a phospho-GSK-3 specific antibody. β-actin was used as a loading control. The densitometric analysis of the protein expression is shown on the bar graph. **p < 0.01 vs. wild-type LEC (n = 3 biologically independent samples for each condition). All statistical analyses were performed using unpaired two-tailed Student’s t-test. All bar graphs are presented as mean ± SEM.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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