IMAGE

Fig 6

ID

ZDB-IMAGE-210802-20

Genes

tagln2

Publication

Wen et al., 2021 - The blood flow-klf6a-tagln2 axis drives vessel pruning in zebrafish by regulating endothelial cell rearrangement and actin cytoskeleton dynamics

All Figures

Figures for Wen et al., 2021

Image

Figure Caption

Fig 6 <italic toggle='yes'>Tagln2</italic> is a direct downstream target of <italic toggle='yes'>klf6a</italic>.

(A) and (B) SiKLF6 in HUVECs efficiently decrease TAGLN2 expression at the mRNA (KLF6: P = 0.0009, TAGLN2: P = 0.0352) and protein (KLF6: P < 0.0001, TAGLN2: P < 0.0001) levels. Student’s unpaired two-tailed t test. *P < 0.05, ***P < 0.001. (C) WISH of the tagln2 gene of sibling and klf6a^-/- zebrafish at 36 hpf. Arrowheads indicate the CVP region expressing tagln2. (D) Structure of the tagln2 promoter. The primer tagln2-F1+R1 contains the conserved Klf6a binding side-CACCC, whereas tagln2-F2+R2 is a negative control located in the tagln2 exon 1. (E) ChIP-PCR of the tagln2 promoter shows that Klf6a can bind directly to the tagln2 promoter.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.