FIGURE

Fig 6

ID

ZDB-FIG-210729-6

Publication

Kowalewski et al., 2021 - Characterization of a member of the CEACAM protein family as a novel marker of proton pump-rich ionocytes on the zebrafish epidermis

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Fig 6

Identification of the ionocyte subtype that expresses CEACAMz1 on zebrafish epidermis.

A-C. High magnification view of the yolk sac surface of a 4 dpf Tg(ceacamz1:mCherry-F) larva (A) immunolabelled with anti-Na⁺/K⁺-ATPase antibody (B); merge of A and B (C). D-F. High magnification view of the yolk sac surface of a 4 dpf Tg(ceacamz1:mCherry-F) larva (D) immunolabelled with anti-vH⁺-ATPase antibody (E); merge of D and E (F). G-I. High magnification view of the yolk sac surface of a 4 dpf Tg(ceacamz1:mCherry-F) larva (G) co-stained with Alexa Fluor 488-conjugated conA (H); merge of G and H (I). (J-L) High magnification view of the gill region of a 22 dpf Tg(ceacamz1:mCherry-F) larva (J) co-stained with Alexa Fluor 488-conjugated conA (K); merge of J and K (L). At all stages analyzed, CEACAMz1-expressing cells perfectly co-localize with cells labelled by the vH⁺-ATPase antibody or by conA. They do not overlap at all with the cells labelled by the Na⁺/K⁺-ATPase antibody.

Expression Data

Genes:	atp6v1b2 mCherry
Antibodies:	Ab1-atp6v1b2 Ab2-atp1a1
Fish:	ump9Tg
Anatomical Terms:	gill vH ionocyte integument vH ionocyte NaK ionocyte vH ionocyte
Stage Range:	Day 4 to Days 21-29

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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