FIGURE

Fig. 9

ID

ZDB-FIG-210726-72

Publication

Lopez-Ramirez et al., 2021 - Inhibition of the HEG1-KRIT1 interaction increases KLF4 and KLF2 expression in endothelial cells

Other Figures

All Figure Page

Back to All Figure Page

Fig. 9

HKi2 treatment increases Akt phosphorylation at S⁴⁷³ that is dependent on PI3K activity. (A-E) hCMEC/D3 cells treated with HKi2 (50 μM) or vehicle control. Cells lysates were analyzed by western blot for Akt and p-Atk S⁴⁷³ protein levels. (A) HKi2 treatment for 1 h activated Akt signaling. (B) HKi2 treatment for 4 h activated Akt signaling and an inactive compound (50 μM), 2-hydroxy-1-naphthoic acid, did not (compound 9). (C) HKi2 treatment for 24 h activated Akt signaling, but the total levels of total Akt were going down. (D,E) Blocking the kinase activity of (E) Akt with MK-2206 (20 μM) and (F) PI3K with LY294002 (10 μM) blunted the activation of Akt by HKi2. (A-H) Bar graphs represent average ± SEM (n = 4), with (A,C) t test, and (B,D,E) one-way ANOVA. *, p < 0.05; **, p < 0.01; ***, p < 0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ FASEB Bioadv