FIGURE

Fig. 6

ID

ZDB-FIG-210726-69

Publication

Lopez-Ramirez et al., 2021 - Inhibition of the HEG1-KRIT1 interaction increases KLF4 and KLF2 expression in endothelial cells

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Fig. 6

HKi2 treatment leads to KLF2 and KLF4 upregulation in endothelial cells. (A-F) hCMEC/D3 cells treated with HKi2 (50 μM) or vehicle control and analyzed by qPCR for mRNA level. (A, B) Dose response of KLF4 and KLF2 mRNA expression at indicated doses for 12 h. HKi2 induces KLF4 and KLF2 mRNA expression at indicated concentrations. (C, D) Timecourse, HKi2 induces a rapid and sustained upregulation of KLF4 and KLF2 mRNA expression. (E,F) HKi2 treatment for 4 h upregulated (E) KLF4, and (F) KLF2, and an inactive compound, 2-hydroxy-1-naphthoic acid (50 μM) (compound 9), did not. (A-F) Bar graphs represent mRNA levels relative to vehicle control ± SEM with: (A-D) n = 3, t test and (E,F) n = 4, one-way ANOVA. *, p < 0.05; **, p < 0.01; ***, p < 0.001. (G) Representative images of hCMEC/D3 cells treated for 12 h with HKi2 (50 μM) or inactive compound (50 μM), 2-hydroxy-1-naphthoic acid, and analyzed by Immunofluorescence for KLF4 protein levels. KLF4 expression is increased after treatment with HKi2. (H) Cell viability as assessed by flow cytometry using Propidium Iodide staining, no significant difference between vehicle, HKi2 and inactive compound, 2-hydroxy-1-naphthoic acid. Percentage of viable cells ± SEM. n=3, one-way ANOVA.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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