ZFIN ID: ZDB-FIG-210710-13
Noy-Lotan et al., 2021 - Cdan1 Is Essential for Primitive Erythropoiesis. Frontiers in Physiology   12:685242 Full text @ Front. Physiol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 8

Loss of cdan1 impairs erythropoiesis in zebrafish embryos. (A) Expression of zebrafish cdan1 at 1 dpf (top) and 2 dpf (bottom). The arrows indicate the expression of cdna1 in the embryonic hematopoietic site. (B) Top, schematic diagram of zebrafish cdan1 gene organization. The binding sites of two morpholinos (MO) are highlighted by red and orange lines. Note zebrafish cdan1 gene contains 28 exons, however, only the first 5 exons are illustrated. Bottom, relative cdan1 expression in wild type (WT) and two cdan1 morphants illustrating that the expression level of cdan1 was strongly reduced upon MO1 or MO2 injection. N indicates the number of biological replicates. (C) Expression pattern of hemoglobin alpha embryonic 1.1. (hbae1.1.) in WT and cdan1 morphants at 1 dpf. The left panel depicts representative images of the hematopoietic tissue. The right depicts the frequency of embryos that show phenotype. Note that injection of control (Ctrl) MO showed a minor effect on hbae1.1. expression. (D)o-dianisidine staining in WT and cdan1 morphants. The left panel depicts representative images of stained embryos 2 dpf. The right panel depicts the frequency of embryos showing phenotype. (E–G) Expression pattern of gata1(E), gata2a(F), and gata2b(G) in the WT and cdan1 morphants. The left panel depicts representative images of the hematopoietic tissue. The right panel depicts the frequency of embryos that show phenotype. N indicates the number of biological samples.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cdan1 WT standard conditions Prim-5 intermediate cell mass of mesoderm ISH
Long-pec intermediate cell mass of mesoderm ISH
gata1a WT standard conditions Long-pec intermediate cell mass of mesoderm ISH
WT + MO1-cdan1 standard conditions Long-pec intermediate cell mass of mesoderm ISH
gata2a WT standard conditions Long-pec intermediate cell mass of mesoderm ISH
WT + MO1-cdan1 standard conditions Long-pec intermediate cell mass of mesoderm ISH
gata2b WT standard conditions Long-pec intermediate cell mass of mesoderm ISH
WT + MO1-cdan1 standard conditions Long-pec intermediate cell mass of mesoderm ISH
hbae1.1 WT standard conditions Prim-5 intermediate cell mass of mesoderm ISH
WT + MO1-cdan1 standard conditions Prim-5 intermediate cell mass of mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-cdan1 standard conditions Prim-5 intermediate cell mass of mesoderm hbae1.1 expression decreased amount, abnormal
Prim-5 primitive erythrocyte differentiation decreased process quality, abnormal
Long-pec intermediate cell mass of mesoderm gata1a expression decreased amount, abnormal
Long-pec intermediate cell mass of mesoderm gata2a expression increased amount, abnormal
Long-pec primitive erythrocyte differentiation decreased process quality, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Physiol.