Loss of cdan1 impairs erythropoiesis in zebrafish embryos. (A) Expression of zebrafish cdan1 at 1 dpf (top) and 2 dpf (bottom). The arrows indicate the expression of cdna1 in the embryonic hematopoietic site. (B) Top, schematic diagram of zebrafish cdan1 gene organization. The binding sites of two morpholinos (MO) are highlighted by red and orange lines. Note zebrafish cdan1 gene contains 28 exons, however, only the first 5 exons are illustrated. Bottom, relative cdan1 expression in wild type (WT) and two cdan1 morphants illustrating that the expression level of cdan1 was strongly reduced upon MO1 or MO2 injection. N indicates the number of biological replicates. (C) Expression pattern of hemoglobin alpha embryonic 1.1. (hbae1.1.) in WT and cdan1 morphants at 1 dpf. The left panel depicts representative images of the hematopoietic tissue. The right depicts the frequency of embryos that show phenotype. Note that injection of control (Ctrl) MO showed a minor effect on hbae1.1. expression. (D)o-dianisidine staining in WT and cdan1 morphants. The left panel depicts representative images of stained embryos 2 dpf. The right panel depicts the frequency of embryos showing phenotype. (E–G) Expression pattern of gata1(E), gata2a(F), and gata2b(G) in the WT and cdan1 morphants. The left panel depicts representative images of the hematopoietic tissue. The right panel depicts the frequency of embryos that show phenotype. N indicates the number of biological samples.