FIGURE

Fig 4

ID

ZDB-FIG-210623-59

Publication

Swartz et al., 2021 - Variation in phenotypes from a Bmp-Gata3 genetic pathway is modulated by Shh signaling

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Fig 4

Gata3 function is required during early palatogenesis.

(A) Embryo carrying the hsp:GATA3-EGFP transgene heat shocked at 48–49 hpf and imaged at 52 hpf expressing human GATA3 in every cell. (B) Flat mount of a cartilage stained 4 dpf hsp:GATA3-EGFP;gata3^+/+ embryo that was heat shocked at 24 hpf with no resulting visible malformations. (C) Flat mount of a cartilage stained 4 dpf neurocranium carrying hsp:GATA3-EGFP;gata3^au42/au42. The mutant embryo was heat shocked at 24 hpf and resulted in a substantial rescue of the trabeculae phenotype. (D) Graph depicting quantification of mutant gata3^au42 phenotype scores at different heat shock time points. Trabeculae phenotype is rescued significantly by hsp:GATA3-EGFP at 24–26 hpf and also at 30 hpf. A one-way ANOVA was performed followed by Dunnett’s multiple comparisons test, gata3^au42/au42 control was compared to all other conditions.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	au34Tg gata3^au42/au42; au34Tg
Condition:	heat shock
Observed In:	neurocranium
Stage:	Day 4

Phenotype Detail

Acknowledgments

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