FIGURE

Figure 2—figure supplement 1.

ID

ZDB-FIG-210414-53

Publication

Willoughby et al., 2021 - The recycling endosome protein Rab25 coordinates collective cell movements in the zebrafish surface epithelium

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Figure 2—figure supplement 1.

MZ<italic>rab25a</italic> and MZ<italic>rab25b</italic> phenotypic characterization.

(A) qPCR analysis of rab25a, rab25b, and rab11a transcripts levels in WT, MZrab25a, and MZrab25b embryos at shield stage. (B) Time-matched phalloidin stained WT and MZrab25b double mutants at 8hpf. Scale bar 100 μm. (C) Tg: MZrab25b (ß-actin;rab25b) epiboly movements rescued compared to MZrab25b mutants. WT (n = 8), MZrab25b (n = 6), and MZrab25b Tg (ß-actin; rab25b) (n = 7) time-matched at 8hpf. Means:SEM; One-Way ANOVA, *p<0.05. (D) Bright-field images of WT and MZrab25b embryos positioned laterally at 3hpf. (E) Stage-matched WT, MZrab25a and MZrab25b embryos at 75% epiboly showing phalloidin staining of F-actin (red) and sytox green labeling of nuclei (green). Embryos positioned laterally with animal pole to the top; Scale bar 100 μm. (F) Whole-mount in situ hybridizations using indicated probes at indicated stages. Scale bar 100 μm. Arrows indicate sox17 staining.

Expression Data

Genes:	gsc krt4 rab11a rab25a rab25b sox17
Fish:	WT rab25a^uot9/uot9 rab25a^uot10/uot10 rab25b^uot11/uot11
Anatomical Terms:	endoderm EVL mesoderm whole organism
Stage Range:	Shield to 75%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rab25a^uot9/uot9 rab25a^uot10/uot10 rab25b^uot11/uot11 rab25b^uot11/uot11; uot16Tg
Observed In:	blastomere DEL epiboly epiboly EVL epiboly forerunner cell group whole organism
Stage Range:	1k-cell to 75%-epiboly

Phenotype Detail

Acknowledgments

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