IMAGE

Figure 2—figure supplement 1.

ID

ZDB-IMAGE-210414-54

Genes

Publication

Willoughby et al., 2021 - The recycling endosome protein Rab25 coordinates collective cell movements in the zebrafish surface epithelium

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Figures for Willoughby et al., 2021

Image

Figure Caption

Figure 2—figure supplement 1. MZ<italic>rab25a</italic> and MZ<italic>rab25b</italic> phenotypic characterization.

(A) qPCR analysis of rab25a, rab25b, and rab11a transcripts levels in WT, MZrab25a, and MZrab25b embryos at shield stage. (B) Time-matched phalloidin stained WT and MZrab25b double mutants at 8hpf. Scale bar 100 μm. (C) Tg: MZrab25b (ß-actin;rab25b) epiboly movements rescued compared to MZrab25b mutants. WT (n = 8), MZrab25b (n = 6), and MZrab25b Tg (ß-actin; rab25b) (n = 7) time-matched at 8hpf. Means:SEM; One-Way ANOVA, *p<0.05. (D) Bright-field images of WT and MZrab25b embryos positioned laterally at 3hpf. (E) Stage-matched WT, MZrab25a and MZrab25b embryos at 75% epiboly showing phalloidin staining of F-actin (red) and sytox green labeling of nuclei (green). Embryos positioned laterally with animal pole to the top; Scale bar 100 μm. (F) Whole-mount in situ hybridizations using indicated probes at indicated stages. Scale bar 100 μm. Arrows indicate sox17 staining.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

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