FIGURE

Fig. 3

ID

ZDB-FIG-210405-3

Publication

Clark et al., 2021 - Complementary and divergent functions of zebrafish Tango1 and Ctage5 in tissue development and homeostasis

Other Figures

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Fig. 3

Trafficking differences in ctage5 vs. tango1 mutant embryos. (A) Representative 40× images showing collagen 2 wholemount immunohistochemistry in 4dpf larvae chondrocytes (n = 3?4 per group). Inserts are 40× images with 3× zoom and cropped to show higher-resolution chondrocyte collagen 2 staining. (B) Representative electron microscopy images of endoplasmic reticulum (*) in 4dpf larvae chondrocytes (n = 3 per group). (C) Representative images and quantification of BODIPY C-12 fluorescence in the intersegmental vessels (arrowheads; one-way ANOVA, F = 39.36, p < 0.0001). (D) Representative images of Oil Red O staining for lipids in 4dpf zebrafish larvae intersegmental vessels (arrowheads). * = p < 0.05, **** = p < 0.0001.

Expression Data

Antibody:	Ab1-col2a
Fish:	WT mia2^mw91/mw91 mia3^mw92/mw92 mia2^mw91/mw91; mia3^mw92/+
Anatomical Terms:	chondrocyte extracellular space chondrocyte intracellular vesicle pharyngeal arch cartilage pharyngeal arch cartilage chondrocyte
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mia2^mw91/mw91 mia3^mw92/mw92 mia2^mw91/mw91; mia3^mw92/+
Observed In:	chondrocyte endoplasmic reticulum intersegmental vessel lipoprotein transport pharyngeal arch cartilage
Stage:	Day 4

Phenotype Detail

Acknowledgments

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