PUBLICATION

Complementary and divergent functions of zebrafish Tango1 and Ctage5 in tissue development and homeostasis

Authors
Clark, E.M., Link, B.A.
ID
ZDB-PUB-210114-16
Date
2021
Source
Molecular biology of the cell   32(5): 391-401 (Journal)
Registered Authors
Link, Brian
Keywords
none
MeSH Terms
  • Animals
  • Endoplasmic Reticulum/metabolism
  • Vesicular Transport Proteins/metabolism
  • Antigens, Neoplasm/genetics
  • Antigens, Neoplasm/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Golgi Apparatus/metabolism
  • Homeostasis
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • COP-Coated Vesicles/metabolism
  • Neoplasm Proteins/genetics*
  • Neoplasm Proteins/metabolism
  • Aryl Hydrocarbon Receptor Nuclear Translocator/genetics*
  • Aryl Hydrocarbon Receptor Nuclear Translocator/metabolism
  • Protein Transport/physiology
  • Carrier Proteins/metabolism
(all 18)
PubMed
33439675 Full text @ Mol. Biol. Cell
Abstract
Coat protein complex II (COPII) factors mediate cargo export from the endoplasmic reticulum (ER), but bulky collagens and lipoproteins are too large for traditional COPII vesicles. Mammalian CTAGE5 and TANGO1 have been well characterized individually as specialized cargo receptors at the ER that function with COPII coats to facilitate trafficking of bulky cargoes. Here, we present a genetic interaction study in zebrafish of deletions in ctage5, tango1, or both to investigate their potential distinct and complementary functions. We found that Ctage5 and Tango1 have different roles related to organogenesis, collagen versus lipoprotein trafficking, stress-pathway activation, and survival. While disruption of both ctage5 and tango1 compounded phenotype severity, mutation of either factor alone revealed novel tissue specific defects in the building of heart, muscle, lens, and intestine, in addition to the previously described roles in the development of neural and cartilage tissues. Together, our results demonstrate that Ctage5 and Tango1 have overlapping functions, but also suggest divergent roles in tissue development and homeostasis.
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
mia2mw91/+standard conditionsDay 4
mia2mw91/+standard conditionsDays 45-89
mia2mw91/+standard conditionsDays 45-89
mia2mw91/+; mia3mw92/+standard conditionsDay 4
mia2mw91/+; mia3mw92/+standard conditionsDays 45-89
mia2mw91/+; mia3mw92/+standard conditionsDays 45-89
mia2mw91/+; mia3mw92/mw92standard conditionsDay 4
mia2mw91/+; mia3mw92/mw92standard conditionsDay 4
mia2mw91/+; mia3mw92/mw92standard conditionsDays 45-89
mia2mw91/+; mia3mw92/mw92standard conditionsDays 45-89
1 - 10 of 74
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
mil1TgTransgenic Insertion
    mw85TgTransgenic Insertion
      mw91
        		Small Deletion
        mw92
          		Small Deletion
          1 - 4 of 4
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          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          mia2CRISPR1-mia2CRISPR
          mia2CRISPR2-mia2CRISPR
          mia3CRISPR1-mia3CRISPR
          mia3CRISPR2-mia3CRISPR
          1 - 4 of 4
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          Fish
          Fish
          mia2mw91/mw91
          mia2mw91/mw91; mia3mw92/mw92
          mia2mw91/mw91; mia3mw92/mw92; mw85Tg
          mia2mw91/mw91; mia3mw92/+
          mia2mw91/mw91; mil1Tg
          mia2mw91/mw91; mw85Tg
          mia2mw91/+
          mia2mw91/+; mia3mw92/mw92
          mia2mw91/+; mia3mw92/+
          mia3mw92/mw92
          1 - 10 of 15
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          Antibodies
          Orthology
          No data available
          Engineered Foreign Genes
          Marker Marker Type Name
          d2EGFPEFGd2EGFP
          EGFPEFGEGFP
          GAL4EFGGAL4
          mCherryEFGmCherry
          1 - 4 of 4
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          Mapping
          No data available
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          Citation
          Clark, E.M., Link, B.A. (2021) Complementary and divergent functions of zebrafish Tango1 and Ctage5 in tissue development and homeostasis. Molecular biology of the cell. 32(5):391-401.