Fig. 4

Stress pathway activation in the nervous system is specific for ctage5 -/- and amplified by tango mutation. (A, A?) Representative images, A, and quantification, A?, of 5XATF6RE:d2GFP activation in 4dpf spinal cords (one-way ANOVA, F = 12.42, p = 0.0005). (B, B?) Representative images of DAPI staining, B, and quantification of brightfield images, B?, in 4dpf spinal cords to show cellular organization (asterisk shows cells mislocalized from the normal lamination pattern; one-way ANOVA, F = 37.51, p < 0.0001). (C, C?) Representative images, C, and quantification, C?, of 5XATF6RE:d2GFP activation in 7dpf spinal cords (one-way ANOVA, F = 22.22, p < 0.0001). (D, D?) Representative images, D, and quantification, D?, of the lysotracker staining area above threshold for acidic compartments in 7dpf spinal cords (one-way ANOVA, F = 3.881, p = 0.0423). (E, E?) Representative images, E, and quantification, E?, of the lysotracker staining area above threshold of lysotracker for acidic compartments in 7dpf brains (one-way ANOVA, F = 2.479, p = 0.1154). (F, F?) Representative images, F, and quantification, F?, of TUNEL staining for cell death in 4dpf brains (one-way ANOVA, F = 24.33, p < 0.0001). (G) Representative images of gap43:GFP in 3?mo old PACT cleared ctage5 wildtype (WT) or homozygous mutant brains. # = p < 0.08, ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001.