Stress pathway activation in the nervous system is specific for ctage5 -/- and amplified by tango mutation. (A, A’) Representative images, A, and quantification, A’, of 5XATF6RE:d2GFP activation in 4dpf spinal cords (one-way ANOVA, F = 12.42, p = 0.0005). (B, B’) Representative images of DAPI staining, B, and quantification of brightfield images, B’, in 4dpf spinal cords to show cellular organization (asterisk shows cells mislocalized from the normal lamination pattern; one-way ANOVA, F = 37.51, p < 0.0001). (C, C’) Representative images, C, and quantification, C’, of 5XATF6RE:d2GFP activation in 7dpf spinal cords (one-way ANOVA, F = 22.22, p < 0.0001). (D, D’) Representative images, D, and quantification, D’, of the lysotracker staining area above threshold for acidic compartments in 7dpf spinal cords (one-way ANOVA, F = 3.881, p = 0.0423). (E, E’) Representative images, E, and quantification, E’, of the lysotracker staining area above threshold of lysotracker for acidic compartments in 7dpf brains (one-way ANOVA, F = 2.479, p = 0.1154). (F, F’) Representative images, F, and quantification, F’, of TUNEL staining for cell death in 4dpf brains (one-way ANOVA, F = 24.33, p < 0.0001). (G) Representative images of gap43:GFP in 3–mo old PACT cleared ctage5 wildtype (WT) or homozygous mutant brains. # = p < 0.08, ** = p < 0.01, *** = p < 0.001, **** = p < 0.0001.
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