Fig. 1
Fig. 1. cdx1b expression during gastrulation and segmentation stages. RNA in situ hybridization was conducted on embryos from 2-cell (A), sphere (B), shield (C), and bud (D, E) stages using a cdx1b antisense RNA probe. Enriched cdx1b expression in the shield (C, arrow) and forebrain neural keel (E) is shown. Double in situ hybridization was performed on embryos from 2 ?s (F) stage using cdx1b (blue) and lft1 (red) RNA probes as well as 24 ?s (H, I) stage using cdx1b (red) and lft1 (green) RNA probes. A sagittal section of stained embryos (G) is shown. Double in situ hybridization was conducted on 22 ?s embryos with cdx1b (blue) and flh (red) probes (J) or with cdx1b (red) and otx5 (green) RNA probes (K). Scale bar (G) represents 100 ??m; scale bar (H, I) represent 40 ??m ?dd, diencephalon; fnk, forebrain neural keel; h, heart; pcp, prechordal plate. |
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Stage Range: | 2-cell to 20-25 somites |
Reprinted from Developmental Biology, 470, Wu, C.S., Lu, Y.F., Liu, Y.H., Huang, C.J., Hwang, S.L., Zebrafish Cdx1b modulates epithalamic asymmetry by regulating ndr2 and lft1 expression, 21-36, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.