Fig. 7. Cdx1b regulates expression of ndr2 and lft1 in the dorsal diencephalon. A Images of a 22?24 ?S embryo that was injected with cdx1b-vivo-MO1 and dextran-fluorescein in its ventral, lateral and dorsal positions. B RNA in situ hybridization was conducted on cdx1b-vivo-4mm MO1-(cdx1b-vivo-4mm) or cdx1b-vivo-MO1-injected embryos to evaluate expression pattern in the dorsal diencephalon using ndr2 RNA probes at 21?22 ?s stage. C RNA in situ hybridization was conducted on cdx1b-vivo-4mm MO1- or cdx1b-vivo-MO1-injected embryos to evaluate expression pattern in the dorsal diencephalon using lft1 RNA probes at 23?24 ?s stage. Quantitative data show the percentages of embryos displaying different expression patterns of ndr2 or lft1 in the dorsal diencephalon in cdx1b-vivo-4mm MO1- and cdx1b-vivo-MO1-injected embryos. Embryos (B, C) are shown in ventral view. Scale bar represents 100 ??m. Chi-squared test of independence was conducted to compare proportions of embryos with absent ndr2 or lft1 expression patterns in the dorsal diencephalon between cdx1b-vivo-4mm MO1- and cdx1b-vivo-MO1-injected embryos. ??, p ?< ?0.01; ns, not significant. dd, diencephalon; fv, forebrain ventricle; hv, hindbrain ventricle; L, left; mv, midbrain ventricle; R, right.
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