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Expression domain of the ventral gene, <italic>eve1</italic>, was significantly reduced in <italic xmlns:xlink='http://www.w3.org/1999/xlink'>zfp665</italic> overexpressed zebrafish embryos.(A-F) WMISH of zebrafish dorsal-ventral genes eve1 (A), vox (B), vent (C), bmp2b (D), gsc (E) and chd (F), in gfp (control) or amphioxus zfp665 mRNA-injected zebrafish embryos. The stages and views of the embryos are indicated below the images. Statistical analyses of eve1, vox, vent, gsc and chd expression domains in embryos injected with gfp and zfp665 mRNA. The expression domains were quantified by measuring the angles of the signals observed from the animal pole. Ectopic expression of vent (arrowhead) was observed in some zfp665-overexpressing embryos (44%, n = 27) (C). The ratio of embryos exhibiting the displayed bmp2b expression patterns is indicated in the bottom right-hand corner (D). V, ventral side; D, dorsal side. Statistical significance was determined by Student’s t-test, ***p < 0.001. Underlying data are available in S1 Data.
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