Figure 3—figure supplement 1.

(A) Pairwise alignment of the mouse and zebrafish TREK1 protein sequences, with the transmembrane domains (TM), extracellular cap (CAP), and selectivity filter (SF) sequences denoted. (B) Representative traces showing temperature dependence of drTREK1 current, measured at temperatures of 20–35°C, in 5°C increments and (C) quantification of temperature responsiveness as measured by drTREK1 current at 0 mV. (D) Representative traces of drTREK1 response to administration of 2 mM Isoflurane or (E) 10 μM BL1249. Experiments performed on mTREK1 channels bearing mutations that alter basal current density, the concentration of microinjected cRNA was titrated to achieve 1 µA of current at 20°C, to approximate wild-type channel current density. (E) Quantification of mTREK1 channel activity on basal current level, (F) temperature dependence as measured by Q10 (30°C/20°C), (G) response to isoflurane administration, (H) changes in external pH, or (I) BL1249 administration, as measured by mTREK1 current at 0 mV. Number of replicate experiments indicated. Error bars are mean ± SEM. Statistically significance was determined in panels F–H by unpaired two tailed t-tests. Results indicated, **p<0.05, ****p<0.0005.