FIGURE

Figure 2—figure supplement 1.

ID

ZDB-FIG-210113-133

Publication

Wague et al., 2020 - Mechanistic insights into volatile anesthetic modulation of K2P channels

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Figure 2—figure supplement 1.

Purification of TREK1 and TRAAK proteins.

(A) Zebrafish TREK1 or human TRAAK DNA sequences were purified by metal chromatography via a C-terminal HIS tag, and expressed as fusion proteins with GFP, cleaved off via a 3C protease cleavage site prior to (B,E) final size exclusion chromatography. (C, F) After SDS PAGE electrophoresis, purified protein ran at a molecular weight of approximately 65 kDa, consistent with a K2P dimer. Prior to photolabeling experiments, purified TREK1 protein was assessed for functional integrity by reconstitution into planar lipid bilayers to measure single-channel activity at the indicated holding potentials. Recordings were performed in symmetrical 150 mM KCl solution (D). hTRAAK protein was similarly active when reconstituted into bilayers (not shown).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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