Figure 2

(A–N) Embryos were collected every 30 min from 2.75 to 7.25 hpf, and transcript levels were quantified using NanoString technology. Error bars represent standard error. Temporal profiles were fit with the cumulative distribution function of the normal distribution (gray lines), and activation time (arrowheads) was defined as the average time point at which the curves reached about two mean average deviations (i.e., 1.5∙τ) from the inflection point ν (excluding the maternally deposited genes id2a [Chong et al., 2005] and smad6a [White et al., 2017]). NanoString probes for two high-confidence activated BMP target genes (apoc1l and znfl2b) were not functional. (O) Average gene expression spatial range is plotted against average activation time. See the Figure 2—source data 1 file for source data.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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