PUBLICATION
Optogenetic investigation of BMP target gene expression diversity
- Authors
- Rogers, K.W., ElGamacy, M., Jordan, B.M., Müller, P.
- ID
- ZDB-PUB-201120-92
- Date
- 2020
- Source
- eLIFE 9: (Journal)
- Registered Authors
- Müller, Patrick, Rogers, Katherine
- Keywords
- cell biology, developmental biology, zebrafish
- Datasets
- GEO:GSE135100
- MeSH Terms
-
- Animals
- Zebrafish/embryology
- Zebrafish/genetics
- Nodal Protein/genetics
- Nodal Protein/metabolism
- Fibroblast Growth Factors/genetics
- Fibroblast Growth Factors/metabolism
- Body Patterning
- Gene Expression Regulation, Developmental*
- Bone Morphogenetic Proteins/genetics*
- Bone Morphogenetic Proteins/metabolism
- Transcription, Genetic
- Kinetics
- Optogenetics*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Signal Transduction
- PubMed
- 33174840 Full text @ Elife
Citation
Rogers, K.W., ElGamacy, M., Jordan, B.M., Müller, P. (2020) Optogenetic investigation of BMP target gene expression diversity. eLIFE. 9:.
Abstract
Signaling molecules activate distinct patterns of gene expression to coordinate embryogenesis, but how spatiotemporal expression diversity is generated is an open question. In zebrafish, a BMP signaling gradient patterns the dorsal-ventral axis. We systematically identified target genes responding to BMP and found that they have diverse spatiotemporal expression patterns. Transcriptional responses to optogenetically delivered high- and low-amplitude BMP signaling pulses indicate that spatiotemporal expression is not fully defined by different BMP signaling activation thresholds. Additionally, we observed negligible correlations between spatiotemporal expression and transcription kinetics for the majority of analyzed genes in response to BMP signaling pulses. In contrast, spatial differences between BMP target genes largely collapsed when FGF and Nodal signaling were inhibited. Our results suggest that, similar to other patterning systems, combinatorial signaling is likely to be a major driver of spatial diversity in BMP-dependent gene expression in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping