PUBLICATION

Optogenetic investigation of BMP target gene expression diversity

Authors
Rogers, K.W., ElGamacy, M., Jordan, B.M., Müller, P.
ID
ZDB-PUB-201120-92
Date
2020
Source
eLIFE   9: (Journal)
Registered Authors
Müller, Patrick, Rogers, Katherine
Keywords
cell biology, developmental biology, zebrafish
Datasets
GEO:GSE135100
MeSH Terms
  • Animals
  • Body Patterning
  • Kinetics
  • Gene Expression Regulation, Developmental*
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Signal Transduction
  • Optogenetics*
  • Fibroblast Growth Factors/genetics
  • Fibroblast Growth Factors/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Nodal Protein/genetics
  • Nodal Protein/metabolism
  • Bone Morphogenetic Proteins/genetics*
  • Bone Morphogenetic Proteins/metabolism
  • Transcription, Genetic
PubMed
33174840 Full text @ Elife
Abstract
Signaling molecules activate distinct patterns of gene expression to coordinate embryogenesis, but how spatiotemporal expression diversity is generated is an open question. In zebrafish, a BMP signaling gradient patterns the dorsal-ventral axis. We systematically identified target genes responding to BMP and found that they have diverse spatiotemporal expression patterns. Transcriptional responses to optogenetically delivered high- and low-amplitude BMP signaling pulses indicate that spatiotemporal expression is not fully defined by different BMP signaling activation thresholds. Additionally, we observed negligible correlations between spatiotemporal expression and transcription kinetics for the majority of analyzed genes in response to BMP signaling pulses. In contrast, spatial differences between BMP target genes largely collapsed when FGF and Nodal signaling were inhibited. Our results suggest that, similar to other patterning systems, combinatorial signaling is likely to be a major driver of spatial diversity in BMP-dependent gene expression in zebrafish.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping