FIGURE

Fig. 7.

ID

ZDB-FIG-200904-8

Publication

Lavergne et al., 2020 - Pancreatic and intestinal endocrine cells in zebrafish share common transcriptomic signatures and regulatory programmes

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Fig. 7.

pax6b inactivation leads to a loss of pancreatic beta cells, reduction of delta cells, increase of epsilon cells and abnormal alpha cells. (Upper panel) percentage of markers from beta-, delta-, alpha-, epsilon- and pan-endocrine cells which are downregulated (in red) or upregulated (in blue) in the pax6b^−/− PECs. The list of markers for each cell subtype is shown in Additional file 9: Table S6. The up- or downregulation was determined from RNA-seq data from pax6b^sa0086 mutant versus wild-type. (Lower panel) Expression analysis of cell subtype markers by WISH (a, b) and FISH (c–x) on 2 dpf zebrafish pax6b mutant and wild-type embryos as noted on the top of the panel; probes are indicated on the left of each picture. Scale bars: 100 µm for panels a and b and 20µm for panels c to x

Expression Data

Genes:	arxa gcga gcgb ghrl ins lamc2 mboat4 ndufa4l2a nkx6.2 nmba pdx1 pnoca scinlb sst2
Fish:	AB pax6b^sa86/sa86
Anatomical Terms:	anterior pancreatic bud endocrine pancreas pancreatic A cell pancreatic B cell pancreatic D cell pancreatic epsilon cell
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pax6b^sa86/sa86
Observed In:	endocrine pancreas pancreatic A cell pancreatic B cell pancreatic D cell pancreatic epsilon cell
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Biol.