ZFIN ID: ZDB-FIG-200806-1
Bonkhofer et al., 2019 - Blood stem cell-forming haemogenic endothelium in zebrafish derives from arterial endothelium. Nature communications   10:3577 Full text @ Nat. Commun.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. S9 Representative fluorescent microscopy images highlighting the DA region of 3 dpf TgBAC(runx1P2:Citrine) embryos either on a runx1+/+ or runx1-/- genetic background. Magnifications highlight the boxed areas. Bottom: ISH analysis of runx1 in the trunk region of 3 dpf runx1+/+ and runx1-/- embryos. Green arrows point to expression in the HE. The region of the caudal haematopoietic tissue (CHT) is indicated. (b) Top and middle: ISH analysis of cmyb in dll4 MO-1 and control embryos co-injected with or without a runx1 over-expression construct. Green arrows point to the HE. Bottom: Expression of runx1 in control and runx1 over-expression embryos. (c) ISH analysis of endothelial genes kdrl, fli1a and cdh5 in control and dll4 MO embryos. Experiment was performed with two different MOs. (d) ISH analysis of runx1 and dll4 expression in control and cldn5b MO embryos

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dll4 WT + MO1-cldn5b standard conditions Prim-5 ventral wall of dorsal aorta ISH
runx1 WT + MO1-cldn5b standard conditions Prim-5 ventral wall of dorsal aorta ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-cldn5b standard conditions Prim-5 ventral wall of dorsal aorta dll4 expression increased amount, abnormal
Prim-5 ventral wall of dorsal aorta runx1 expression decreased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.