ZFIN Figure: Bonkhofer et al., 2019, Fig. S1

ZFIN ID: ZDB-FIG-190910-31

ZFIN ID: ZDB-FIG-190910-31

Bonkhofer et al., 2019 - Blood stem cell-forming haemogenic endothelium in zebrafish derives from arterial endothelium. Nature communications 10:3577 Full text @ Nat. Commun.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Gene:	Citrine
Fish:	ox1Tg ox1Tg + MO1-gata2b ox1Tg + MO8-tal1 ox1Tg; s896Tg
Knockdown Reagents:	MO1-gata2b MO8-tal1
Anatomical Terms:	caudal hematopoietic tissue kidney lateral plate mesoderm posterior lateral plate mesoderm spinal cord neuron thymus ventral wall of dorsal aorta
Stage Range:	1-4 somites to Day 6

PHENOTYPE:

Fish:	ox1Tg + MO1-gata2b ox1Tg + MO8-tal1
Knockdown Reagents:	MO1-gata2b MO8-tal1
Observed In:	ventral wall of dorsal aorta
Stage:	Prim-15

Fig. S1

Proper regulation of Citrine expression in TgBAC(runx1P2:Citrine) embryos (a) Multiple sequence alignment between the 5’ most bases of the runx1 alternative promoter products from human (hRUNX1) and zebrafish (zRunx1). P1 specific sequences are shown in red. The zebrafish 2a specific sequence is shown in grey. The in-exon splice site for the human transcript is highlighted in yellow. (b) Schematic representation of the zebrafish runx1 locus and regions covered by each of the previously identified zebrafish BACs1. (c) ISH for runx1 and Citrine in early TgBAC(runx1P2:Citrine) embryos depicting the region of the posterior lateral mesoderm (PLM). (d) Microscopic images of Citrine fluorescence focusing on the PLM region. Orange arrows indicate region shown in the top view panel. (e) ISH for runx1 in WT and early TgBAC(runx1P2:Citrine) embryos at 29 hpf. (f) Fluorescent microscopy image of a 50 hpf double transgenic TgBAC(runx1P2:Citrine);Tg(kdrl:mCherry) embryo focusing on the trunk region. (g) Representative microscopic image of a 6 dpf TgBAC(runx1P2:Citrine) embryo. The region of the thymus (Th), kidney (Ki) and caudal haematopoietic tissue (CHT) are indicated. (h) ISH for Citrine or runx1 in the trunk region of embryos injected with MOs targeting tal1b or gata2b. ISH for kdrl controls for integrity of vascular structures.

Gene Expression Details

Gene	Fish	Conditions	Stage	Anatomy	Assay
Citrine	ox1Tg	standard conditions	1-4 somites	lateral plate mesoderm	ISH
			5-9 somites	posterior lateral plate mesoderm	IFL
			5-9 somites	posterior lateral plate mesoderm	ISH
			14-19 somites	posterior lateral plate mesoderm	IFL
			Prim-5	ventral wall of dorsal aorta	ISH
			Prim-15	ventral wall of dorsal aorta	ISH
			Day 6	caudal hematopoietic tissue	IFL
			Day 6	kidney	IFL
			Day 6	spinal cord neuron	IFL
			Day 6	thymus	IFL
	ox1Tg + MO1-gata2b	standard conditions	Prim-15	ventral wall of dorsal aorta	ISH
	ox1Tg + MO8-tal1	standard conditions	Prim-15	ventral wall of dorsal aorta	ISH
	ox1Tg; s896Tg	standard conditions	Long-pec	spinal cord neuron	IFL
			Long-pec	ventral wall of dorsal aorta	IFL

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
ox1Tg + MO1-gata2b	standard conditions	Prim-15	ventral wall of dorsal aorta Citrine expression decreased amount, abnormal
ox1Tg + MO8-tal1	standard conditions	Prim-15	ventral wall of dorsal aorta Citrine expression decreased amount, abnormal

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.