ZFIN ID: ZDB-FIG-190910-31
Bonkhofer et al., 2019 - Blood stem cell-forming haemogenic endothelium in zebrafish derives from arterial endothelium. Nature communications   10:3577 Full text @ Nat. Commun.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 1-4 somites to Day 6
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-15

Fig. S1

Proper regulation of Citrine expression in TgBAC(runx1P2:Citrine) embryos (a) Multiple sequence alignment between the 5’ most bases of the runx1 alternative promoter products from human (hRUNX1) and zebrafish (zRunx1). P1 specific sequences are shown in red. The zebrafish 2a specific sequence is shown in grey. The in-exon splice site for the human transcript is highlighted in yellow. (b) Schematic representation of the zebrafish runx1 locus and regions covered by each of the previously identified zebrafish BACs1. (c) ISH for runx1 and Citrine in early TgBAC(runx1P2:Citrine) embryos depicting the region of the posterior lateral mesoderm (PLM). (d) Microscopic images of Citrine fluorescence focusing on the PLM region. Orange arrows indicate region shown in the top view panel. (e) ISH for runx1 in WT and early TgBAC(runx1P2:Citrine) embryos at 29 hpf. (f) Fluorescent microscopy image of a 50 hpf double transgenic TgBAC(runx1P2:Citrine);Tg(kdrl:mCherry) embryo focusing on the trunk region. (g) Representative microscopic image of a 6 dpf TgBAC(runx1P2:Citrine) embryo. The region of the thymus (Th), kidney (Ki) and caudal haematopoietic tissue (CHT) are indicated. (h) ISH for Citrine or runx1 in the trunk region of embryos injected with MOs targeting tal1b or gata2b. ISH for kdrl controls for integrity of vascular structures.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
Citrine ox1Tg standard conditions 1-4 somites lateral plate mesoderm ISH
5-9 somites posterior lateral plate mesoderm ISH
5-9 somites posterior lateral plate mesoderm IFL
14-19 somites posterior lateral plate mesoderm IFL
Prim-5 ventral wall of dorsal aorta ISH
Prim-15 ventral wall of dorsal aorta ISH
Day 6 caudal hematopoietic tissue IFL
Day 6 kidney IFL
Day 6 spinal cord neuron IFL
Day 6 thymus IFL
ox1Tg + MO1-gata2b standard conditions Prim-15 ventral wall of dorsal aorta ISH
ox1Tg + MO8-tal1 standard conditions Prim-15 ventral wall of dorsal aorta ISH
ox1Tg; s896Tg standard conditions Long-pec spinal cord neuron IFL
Long-pec ventral wall of dorsal aorta IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ox1Tg + MO1-gata2b standard conditions Prim-15 ventral wall of dorsal aorta Citrine expression decreased amount, abnormal
ox1Tg + MO8-tal1 standard conditions Prim-15 ventral wall of dorsal aorta Citrine expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Nature communications for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nat. Commun.