ZFIN ID: ZDB-FIG-190910-27
Bonkhofer et al., 2019 - Blood stem cell-forming haemogenic endothelium in zebrafish derives from arterial endothelium. Nature communications   10:3577 Full text @ Nat. Commun.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-5

Fig. 5

Expression of dll4 in aortic HE is repressed by Runx1. ab qRT-PCR gene expression analysis of NOTCH ligands jag1a and dll4 in the HE and ARE of runx1+/+ (WT) and the HE of runx1−/− mutant (MUT) embryos. Graphs in (a), (b) and (e) show the mean of detected expression levels relative to the geometric mean (GM) of the two housekeeping genes eef1a1l and rpl13an = 5 independent biological experiments for WT embryos and n = 6 independent biological experiments for MUT embryos. Error bars represent the SEM. two-way ANOVA; *p < 0.05; **p < 0.01; ***p < 0.001. c Spatial analysis of gene expression of dll4 in runx1+/+ and runx1−/− embryos by ISH. Green arrows point to the HE. Orange arrows point to the DA roof. d Maximum intensity projection of representative confocal images of runx1+/+ and runx1−/− embryos with double FISH for dll4 and runx1. Red arrows in the close-up highlight the region of a runx1+ cell. e qRT-PCR gene expression analysis of sox17 in the HE and ARE of runx1+/+ (WT) and the HE of runx1−/− mutant (MUT) embryos. f ISH analysis of sox17 in runx1+/+ and runx1−/− embryos. Green arrows point to the HE

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dll4 runx1hg1/hg1 standard conditions Prim-5 ventral wall of dorsal aorta ISH
sox17 WT standard conditions Prim-5 Not Detected ventral wall of dorsal aorta ISH
runx1hg1/hg1 standard conditions Prim-5 ventral wall of dorsal aorta ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
runx1hg1/hg1 standard conditions Prim-5 ventral wall of dorsal aorta dll4 expression increased amount, abnormal
Prim-5 ventral wall of dorsal aorta sox17 expression increased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.