Fig. 5.

Macrophages phagocytose and degrade Toxoplasma gondii in vivo. (A) Representative frames extracted from in vivo confocal imaging of mpeg1:G/U:mCherry larvae harboring macrophages (red) injected with type I Toxoplasma-GFP (green). First frame at 2 h 12 min post-infection (mpi) followed by seven consecutive frames taken at 8 min intervals. Shown are maximum projections of 24 z-slices taken at 2 µm optical sections. White arrowheads label a phagocytosed parasite at 2 h 20 mpi that loses its green fluorescence by 3 h 8 mpi. Yellow arrowheads indicate a new phagocytosis event of a green parasite at 3 hpi to 3 h 8 mpi. Scale bar: 10 µm. See also Movie 5. (B) 3D CLEM of dead/dying tachyzoites in the HBV of mpeg1:G/U:mCherry larvae harboring macrophages (red) infected with type I Toxoplasma-GFP (green) at 6 hpi and stained with Hoechst 33342 (blue). Representative images extracted from confocal z-stacks of a full vibratome section (left column) and from consecutive 50 nm SBF SEM slices of a segment of it (right column). Dead/dying parasites are indicated by white arrowheads (insets, left column) and outlined by green dashed lines (right column). Scale bars: 10 µm (left column) and 1 µm (right column).