Fig. 4.

Leukocyte recruitment to Toxoplasma gondii in vivo. (A) Representative images of mpeg1:G/U:mCherry larvae harboring macrophages (red) infected in the HBV with Toxoplasma (green). Individual larvae were imaged and monitored at 0, 6 and 24 hpi by fluorescent stereomicroscopy. Scale bar: 100 µm. (B) Quantification of macrophages in mpeg1:G/U:mCherry larvae at 0, 6 and 24 hpi injected with mock [human foreskin fibroblast (HFF) lysate; gray open circles], type I (RH; open circles), type II (Pru; semi-closed circles) or type III (CEP; closed circles) parasites. Pooled data from at least three independent experiments with at least seven larvae per condition per experiment. Mean±s.e.m. shown. Significance calculated using two-way ANOVA (repeated measures) with Sidak's multiple comparisons test. ns, P>0.05; ***P≤0.001. p.i., post-infection. (C) Representative images of lyz:dsRed larvae harboring neutrophils (red) infected in the HBV with Toxoplasma (green). Individual larvae were imaged and monitored at 0, 6 and 24 hpi by fluorescent stereomicroscopy. Scale bar: 100 µm. (D) Quantification of neutrophils in lyz:dsRed larvae at 0, 6 and 24 hpi injected with mock (HFF lysate; gray open circles), type I (RH; open circles), type II (Pru; semi-closed circles) or type III (CEP; closed circles). Pooled data from at least two independent experiments with at least three larvae per condition per experiment. Mean±s.e.m. shown. Significance calculated using two-way ANOVA (repeated measures) with Tukey's multiple comparisons test. ns, P>0.05; *P≤0.01, **P≤0.01, ***P≤0.001.