Protein partners of zebrafish Hspb8. (A) Western blot analysis of co-immunoprecipitation (co-IP) assay results. Each experiment involved the use of three columns: the first containing resin with an immobilized anti-Hspb8 antibody, the second an immobilized anti-Bag3 antibody, and the third only resin was used as a negative control. Hspb8 and Bag3 interact with each other. Moreover, Bag3 interacts with LC3 I/II. (B) and (C), List of peptides representing proteins involved in the formation of the autophagy-inducing complex detected by LC-MS analysis of eluates obtained from the co-IP experiment conducted using the column with an immobilized anti-Bag3 antibody (B), anti-Hspb8 antibody (C). (D) and (E), List of peptides representing Hspb1 detected by LC-MS analysis of eluates obtained from the co-IP experiment conducted using the column with an immobilized anti-Bag3 antibody (D) and anti-Hspb8 antibody (E). (F) List of peptides representing neuronal proteins detected by LC-MS analysis of eluates obtained from the co-IP experiment conducted using the column with an immobilized anti-Hspb8 antibody.
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