PUBLICATION
Characterization of Hspb8 in Zebrafish
- Authors
- Dubińska-Magiera, M., Niedbalska-Tarnowska, J., Migocka-Patrzałek, M., Posyniak, E., Daczewska, M.
- ID
- ZDB-PUB-200702-2
- Date
- 2020
- Source
- Cells 9(6): (Journal)
- Registered Authors
- Keywords
- Bag3, Hspb1, Hspb8, autophagy, muscle, sHSP, zebrafish
- MeSH Terms
-
- Animals
- Autophagy
- Heat-Shock Proteins/genetics*
- Molecular Chaperones/genetics*
- Zebrafish
- PubMed
- 32604890 Full text @ Cells
Citation
Dubińska-Magiera, M., Niedbalska-Tarnowska, J., Migocka-Patrzałek, M., Posyniak, E., Daczewska, M. (2020) Characterization of Hspb8 in Zebrafish. Cells. 9(6):.
Abstract
Hspb8 is a member of the small heat shock protein (sHSP) family. Its expression is known to be upregulated under heat shock. This protein interacts with different partners and can, therefore, be involved in various processes relevant to tissue integrity and functioning. In humans, mutations in the gene encoding Hspb8 can lead to the development of various diseases such as myopathies and neuropathies. In our study, we aimed to perform an in-depth characterization of zebrafish Hspb8 during zebrafish development. We applied techniques such as RT-qPCR, Western blot, immunofluorescence, co-immunoprecipitation, LC-MS, and morpholino-mediated knockdown. We broadened the knowledge regarding zebrafish hspb8 expression during development under normal and heat shock conditions as well as its tissue- and subcellular-specific localization. A co-IP analysis allowed us to conclude that zebrafish Hspb8 can interact with proteins such as Bag3 and Hsc70, which are crucial for formation of an autophagy-inducing complex. We also demonstrated that hspb8 morpholino-mediated knockdown has an impact on zebrafish embryos' morphology, muscle ultrastructure, and motility behavior. Our research provides a valuable resource for the potential use of the zebrafish as a model for studying pathological conditions associated with hspb8 disorders.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping