Tissue-specific expression and localization of Hspb8 during zebrafish development under normal and heat shock conditions. A and B. Real-time quantitative PCR (RT qPCR) of hspb8 mRNA expression level during zebrafish development (from 1 to 5 dpf) in normal (upper part A and B) and heat shock conditions (bottom part A and B). Expression of hspb8 mRNA was normalized to eef1a1l1 (A) and rpl13a (B). Error bars show the standard deviation. The tables below indicate the pairwise comparison between hspb8 expression level during zebrafish development (from 1 to 5 dpf) under normal and heat shock conditions. Statistically significant differences are indicated with *; * p < 0.05 and ** p < 0.001 (Student’s t-test). The experiment was performed 3 times, n = 25–30. A.U. = arbitrary unit. (C) Cross-sections of the mid-trunk region of 48 and 72 hpf zebrafish embryos under normal and heat shock conditions. The Hspb8 (red) localizes in muscle cells (yellow arrowheads) and the lateral spinal cord (white arrowheads). α-Actinin (green) is used to show muscles. Nuclei are stained blue. Cryosection (14 μm thick). Images represent single cross-sections of the mid-trunk region (0.45 μm thick). Scale bar: 10 μm. Images were obtained with a confocal laser scanning microscope (Olympus FV1000). (D) hspb8 expression in 48 and 60 hpf zebrafish embryos under heat shock conditions, whole-mount in situ hybridization. The hspb8 mRNA localizes in muscles (yellow arrowheads) and the lateral spinal cord (red arrowheads).
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