FIGURE

Fig. s3

ID

ZDB-FIG-200611-32

Publication

Santos-Ledo et al., 2020 - Alternative splicing of jnk1a in zebrafish determines first heart field ventricular cardiomyocyte numbers through modulation of hand2 expression

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Fig. s3

WISH for all jnk1a and jnk1b alternatively spliced transcripts. Chromogenic WISH is not quantitative but indicates the extent of expression. (A) Prior to heart formation all transcripts are present throughout the embryo at the start of gastrulation (6hpf) and the end of gastrulation (10hpf). The most abundant transcripts by RT-PCR splicing assay are indicated by dashed lines. (B) WISH for all jnk1a and jnk1b alternatively spliced transcripts at 24hpf and 48hpf. Transcripts with obvious expression within heart—jnk1a Ex7Lg and jnk1b Ex8Sh—are indicated by arrows. (C) Close up of heart in all jnk1a transcripts at 24 and 48hpf. Outline of heart indicated by dashed lines. Absent expression indicated by arrow heads and expression in heart indicated by arrows in jnk1a Ex7Lg, jnk1a Ex8Lg and jnk1b Ex8Sh. (D) WISH demonstrated expression of jnk2 and jnk3 in the developing brain (arrows) but not heart (arrowheads). (E) RT-PCR of extracted hearts indicates that jnk2 is expressed at low level at 24 and 48 hpf, whilst there is late onset of jnk3 gene expression by 48hpf. ef1alpha is used as a loading control.

Expression Data

Genes:	mapk8a mapk8b mapk9 mapk10
Fish:	WT
Anatomical Terms:	brain heart heart tube whole organism
Stage Range:	Shield to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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