FIGURE

Fig 2

ID

ZDB-FIG-200610-23

Publication

Santos-Ledo et al., 2020 - Alternative splicing of jnk1a in zebrafish determines first heart field ventricular cardiomyocyte numbers through modulation of hand2 expression

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Fig 2

Semi-quantitative <italic>jnk1a/b</italic> transcript assay.

(A) Schematic of assay for jnk1a (see methods for full details). RNA is extracted and a cDNA pool produced. PCR within linear phase performed using forward (fwd) primers specific for jnk1a/1b exons 7/8 and common reverse (rev) primers. The fraction with C-terminal extension is determined by restriction enzyme (RE) digestion. (B) Percentage of total jnk1a and jnk1b transcripts during embryonic development. 14 ss = 16 hpf. (C) Percentage of total Lg jnk1a and jnk1b transcripts during embryonic development. (D and D') Proportion of each individual alternatively spliced transcript during development in whole embryo. (E and E') Proportion of each individual transcript within isolated whole heart during development. jnk1a = blue, jnk1b = green, Ex7 = circle, Ex8 = square, Sh = outline black and Lg = no outline. n = 6–8 at all time points.

Expression Data

Genes:	mapk8a mapk8b
Fish:	WT
Anatomical Terms:	heart heart tube whole organism
Stage Range:	1-cell to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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