Figure 6
- ID
- ZDB-FIG-200530-47
- Publication
- Ye et al., 2020 - Yap-lin28a axis targets let7-Wnt pathway to restore progenitors for initiating regeneration
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(A) We created hs:let7 transgenic line and found that it recapitulates the phenotype of lin28a-/- by decreasing proliferative SCs post LY+neo. (B) The induction of EdU+ proliferative cells was blocked by hs:let7, indicating that let7 microRNA acts downstream of lin28a to induce proliferation. (C) In situ hybridization results showed that expression of Wnt pathway genes, such as wnt10a and mycn, were increased in hs:lin28a. The activation of Wnt pathway genes in hs:lin28a were blocked when let7 was overexpressed, indicating that let7 acts downstream of lin28a to inhibit Wnt pathway. (D) Expressions of Wnt pathway genes, such as mycn, wnt10a and ccnd1 (cyclind1), were highly induced at 1 hr post LY+neo in sibling, but were not detected in lin28a-/-. (E) Inhibition of Wnt pathway with hs:dkk1 decreased lin28a-induced EdU+ proliferative cells, indicating that Wnt activation acts downstream of lin28a to induce regeneration. |
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| Stage: | Day 5 |
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| Stage: | Day 5 |