PUBLICATION
Yap-lin28a axis targets let7-Wnt pathway to restore progenitors for initiating regeneration
- Authors
- Ye, Z., Su, Z., Xie, S., Liu, Y., Wang, Y., Xu, X., Zheng, Y., Zhao, M., Jiang, L.
- ID
- ZDB-PUB-200501-4
- Date
- 2020
- Source
- eLIFE 9: (Journal)
- Registered Authors
- Jiang, Linjia
- Keywords
- developmental biology, zebrafish
- MeSH Terms
-
- Animals
- Cell Proliferation/physiology
- Ear, Inner/metabolism
- Gene Expression Regulation, Developmental/physiology
- Hair Cells, Auditory/physiology
- Lateral Line System/metabolism*
- Nerve Regeneration/physiology*
- Receptors, Notch/metabolism*
- Regeneration/physiology*
- Transcription Factors/metabolism
- Zebrafish/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 32352377 Full text @ Elife
Citation
Ye, Z., Su, Z., Xie, S., Liu, Y., Wang, Y., Xu, X., Zheng, Y., Zhao, M., Jiang, L. (2020) Yap-lin28a axis targets let7-Wnt pathway to restore progenitors for initiating regeneration. eLIFE. 9:.
Abstract
The sox2 expressing (sox2+) progenitors in adult mammalian inner ear lose the capacity to regenerate while progenitors in the zebrafish lateral line are able to proliferate and regenerate damaged HCs throughout lifetime. To mimic the HC damage in mammals we have established a zebrafish severe injury model to eliminate both progenitors and HCs. The atoh1a expressing (atoh1a+) HC precursors were the main population that survived post severe injury, and gained sox2 expression to initiate progenitor regeneration. In response to severe injury, yap was activated to upregulate lin28a transcription. Severe-injury-induced progenitor regeneration was disabled in lin28a or yap mutants. In contrary, overexpression of lin28a initiated the recovery of sox2+ progenitors. Mechanistically, microRNA let7 acted downstream of lin28a to activate Wnt pathway for promoting regeneration. Our findings that lin28a is necessary and sufficient to regenerate the exhausted sox2+ progenitors shed light on restoration of progenitors to initiate HC regeneration in mammals.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping