FIGURE

Figure 3—figure supplement 1.

ID

ZDB-FIG-200523-36

Publication

Koizumi et al., 2016 - The aspartyl protease DDI2 activates Nrf1 to compensate for proteasome dysfunction

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Figure 3—figure supplement 1.

Genome editing of <italic>DDI2</italic> locus by CRISPR-Cas9 system.

(A) Schematic diagram of DDI2 wild-type (WT), knockout (KO), and knock-in (KI) alleles. Red arrowhead indicates sgRNA targeting site, and blue arrows indicate PCR primers for confirmation of homologous recombination. puro^R indicates a puromycin resistant cassette. (B) Confirmation of successful genome editing at the DDI2 locus. The genome DNA of each cell line was extracted and amplified by PCR using primer pairs shown in (A).

DOI:http://dx.doi.org/10.7554/eLife.18357.009

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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