FIGURE

Figure 1—figure supplement 1.

ID

ZDB-FIG-200523-31

Publication

Koizumi et al., 2016 - The aspartyl protease DDI2 activates Nrf1 to compensate for proteasome dysfunction

Other Figures

All Figure Page

Back to All Figure Page

Figure 1—figure supplement 1.

Methods for the genome-wide screen.

(A) A schematic view of the workflow in the primary screen using HEK293A cells. (B) Definition of nucleus and cytoplasm in the screen. Region of interest (ROI) of the nucleus was defined as a circle drawn two pixels inside of the outermost DAPI signal. ROI of the cytoplasm was defined as a three-pixel wide ring around DAPI signal. No gap between the rings and DAPI signals was set in the analysis using HEK293A cells, whereas a one-pixel gap was set in the analysis using HT1080 cells. (C) The well score of negative (no siRNA) and positive (si p97) controls. The well score is the fluorescence intensity ratio of ROI of nucleus to cytoplasm as defined in (B). The Z’ factor was calculated from 12 individual wells on each plate.

DOI:http://dx.doi.org/10.7554/eLife.18357.005

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife