IMAGE

Figure 3—figure supplement 1.

ID: ZDB-IMAGE-200523-36
Source: Figures for Koizumi et al., 2016

Image

Figure Caption

Figure 3—figure supplement 1. Genome editing of <italic>DDI2</italic> locus by CRISPR-Cas9 system.

(A) Schematic diagram of DDI2 wild-type (WT), knockout (KO), and knock-in (KI) alleles. Red arrowhead indicates sgRNA targeting site, and blue arrows indicate PCR primers for confirmation of homologous recombination. puro^R indicates a puromycin resistant cassette. (B) Confirmation of successful genome editing at the DDI2 locus. The genome DNA of each cell line was extracted and amplified by PCR using primer pairs shown in (A).

DOI:http://dx.doi.org/10.7554/eLife.18357.009

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife