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Figure 1—figure supplement 1.

ID: ZDB-IMAGE-200523-31
Source: Figures for Koizumi et al., 2016

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Figure Caption

Figure 1—figure supplement 1. Methods for the genome-wide screen.

(A) A schematic view of the workflow in the primary screen using HEK293A cells. (B) Definition of nucleus and cytoplasm in the screen. Region of interest (ROI) of the nucleus was defined as a circle drawn two pixels inside of the outermost DAPI signal. ROI of the cytoplasm was defined as a three-pixel wide ring around DAPI signal. No gap between the rings and DAPI signals was set in the analysis using HEK293A cells, whereas a one-pixel gap was set in the analysis using HT1080 cells. (C) The well score of negative (no siRNA) and positive (si p97) controls. The well score is the fluorescence intensity ratio of ROI of nucleus to cytoplasm as defined in (B). The Z’ factor was calculated from 12 individual wells on each plate.

DOI:http://dx.doi.org/10.7554/eLife.18357.005

Acknowledgments

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