FIGURE

Figure 8.

ID

ZDB-FIG-200316-8

Publication

Hung et al., 2020 - Wnt5b integrates Fak1a to mediate gastrulation cell movements via Rac1 and Cdc42

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Figure 8.

Fak1a and Wnt5b reciprocally rescue convergence defects in wnt5b and fak1a MO-injected embryos, respectively. (a,b) Embryos were untreated, injected with 7.5 ng of the wnt5b MO only or co-injected with both 7.5 ng of the wnt5b MO and 200 pg of fak1a mRNA. (c,d) Embryos were untreated, injected with 5 ng of the fak1a tMO₁ only, or co-injected with both 5 ng of the fak1a tMO₁ and 10 pg of wnt5b mRNA. Embryos were then cultured to the bud stage and subjected to WISH against ctsl1b, dlx3 and ntl to analyse convergence and extension as described in figure 4. Representative photographs are shown in (a) and (c), and quantitative analyses are shown in (b) and (d) (n = 3, **p < 0.01, ***p < 0.001).

Expression Data

Genes:	ctslb dlx3b tbxta
Fish:	WT WT + MO1-wnt5b WT + MO6-ptk2ab
Knockdown Reagents:	MO1-wnt5b MO6-ptk2ab
Anatomical Terms:	ectoderm notochord prechordal plate
Stage:	Bud

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-wnt5b WT + MO6-ptk2ab
Knockdown Reagents:	MO1-wnt5b MO6-ptk2ab
Observed In:	convergent extension
Stage:	Bud

Phenotype Detail

Acknowledgments

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