ZFIN ID: ZDB-FIG-200314-87
Bryan et al., 2020 - Optic cup morphogenesis requires neural crest-mediated basement membrane assembly. Development (Cambridge, England)   147(4) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 7.

Nidogen is required for optic cup morphogenesis. (A-H) Whole-mount in situ hybridization for nidogens in 24 hpf control (A-D) and MZnid1b; MZnid2a mutant (E-H) embryos. Insets show magnified view of head. (I-T) Optic cup morphology and neural crest localization in EGFP-CAAX mRNA-injected, Tg(sox10:memRFP)+ embryos: control (I,M,Q), MZnid1b;MZnid2a mutant (J,N,R), golden crispant MZnid1b;MZnid2a mutant (K,O,S) and nid1a;nid2b crispant MZnid1b;MZnid2a mutant (L,P,T). (U) RT-qPCR quantification of nidogen transcripts in MZnid1b;MZnid2a mutant embryos, normalized to wild-type control expression levels (magenta dashed line, NRQ=1). Results from three biological replicates run in triplicate. (V-X) Quantification of invagination angle (V), optic fissure angle (W) and lens roundness (X). n (embryos) shown in base of graph. P-values were calculated using one-way ANOVA with Tukey HSD post-hoc test. Scale bar: 50 μm. D, dorsal; L, lateral; M, medial; N, nasal; T, temporal; V, ventral.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
nid1a nid1bsa13713/sa13713; nid2asa15802/sa15802 standard conditions Prim-5 head ISH
Prim-5 whole organism RTPCR
nid1b nid1bsa13713/sa13713; nid2asa15802/sa15802 standard conditions Prim-5 head ISH
nid2a nid1bsa13713/sa13713; nid2asa15802/sa15802 standard conditions Prim-5 head ISH
nid2b nid1bsa13713/sa13713; nid2asa15802/sa15802 standard conditions Prim-5 head ISH
Prim-5 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nid1bsa13713/sa13713; nid2asa15802/sa15802 standard conditions Prim-5 head nid1a expression decreased amount, abnormal
Prim-5 head nid2b expression increased amount, abnormal
Prim-5 whole organism nid1a expression decreased amount, abnormal
Prim-5 whole organism nid2b expression increased amount, abnormal
nid1bsa13713/sa13713; nid2asa15802/sa15802; vu234Tg + CRISPR3-nid1a + CRISPR3-nid2b control Prim-5 closure of optic fissure decreased occurrence, abnormal
Prim-5 optic cup morphogenesis involved in camera-type eye development process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development