FIGURE

Fig. 2

ID

ZDB-FIG-200302-55

Publication

Librán-Pérez et al., 2019 - Antiviral activity of palmitic acid via autophagic flux inhibition in zebrafish (Danio rerio)

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Fig. 2

Measurement of different immune parameters after PA treatment. (A) Effect of PA and SVCV infection in the apoptosis of ZF4 cells measured by flow cytometry. Data represent the mean of four independent experiments ± SEM. (B, C) Relative mRNA abundance of ifnphi1 (B) and ifnphi2(C) in pools of 4–5 zebrafish larvae treated or not with PA in the absence or presence of SVCV infection. The data represent the mean ± SEM of 5–6 measurements. (D, E) Neutrophil count in the transgenic Mpo:GFP zebrafish larvae treated with PA for 24 h and then infected or not with SVCV. The numbers in the images represent the animals with the shown phenotype per total analysed larvae. The neutrophil count is presented as the mean ± SEM of the analysed fish by treatment. Comparisons among groups were made using one-way ANOVA (multiple comparisons) followed by a Student-Newman-Keuls test. Significant differences are displayed as *** (0.0001 < p < 0.001), ** (0.001 < p < 0.01) or * (0.01 < p < 0.05).

Expression Data

Genes:	GFP ifnphi1 ifnphi2
Fish:	WT i114Tg
Conditions:	chemical treatment by environment: hexadecanoic acid chemical treatment by environment: hexadecanoic acid, viral treatment by exposure to environment: Sprivivirus cyprinus viral treatment by exposure to environment: Sprivivirus cyprinus
Anatomical Terms:	neutrophil whole organism
Stage Range:	Day 5 to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT i114Tg
Conditions:	chemical treatment by environment: hexadecanoic acid chemical treatment by environment: hexadecanoic acid, viral treatment by exposure to environment: Sprivivirus cyprinus viral treatment by exposure to environment: Sprivivirus cyprinus
Observed In:	neutrophil whole organism
Stage Range:	Day 5 to Days 7-13

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Fish Shellfish Immunol.