Fig. 1-S1
- ID
- ZDB-FIG-200106-6
- Publication
- Jiang et al., 2019 - A conserved morphogenetic mechanism for epidermal ensheathment of nociceptive sensory neurites
- Other Figures
-
- Fig. 1
- Fig. 1-S1
- Fig. 1-S2
- fig2
- fig3
- Figure 2—figure supplement 1.
- Figure 3—figure supplement 1.
- Figure 4
- Figure 4—figure supplement 1.
- Figure 4—figure supplement 2.
- Figure 4—figure supplement 3.
- Figure 5
- Figure 5—figure supplement 1.
- Figure 5—figure supplement 2.
- Figure 5—figure supplement 3.
- Figure 6
- Figure 6—figure supplement 1.
- Figure 6—figure supplement 3.
- Figure 7—figure supplement 1.
- All Figure Page
- Back to All Figure Page
Screen for epithelial markers that accumulate at sites of c4da dendrite contact.( A) Summary of screen results. For a full list of markers screened, see Supplementary file 1. ( B) Co-localization of PLCδ-PH-GFP and coracle. Maximum intensity projections of GFP, cora, and HRP (to label PNS neurons) immunostaining of larva expressing PLCδ-PH-GFP in the epidermis. Bracket marks glial sheaths labeled by cora. ( C) PH domain mutation abrogates PLCδ-PH-GFP sheath labeling. Image shows epidermal distribution of a PLCδ-PH-GFP mutant (S39R) that is unable to bind PIP2 in a larva expressing the c4da-specific marker ppk-CD4-tdTomato. ( D) PIP2 is locally depleted from branch points. Plot depicts ratios of PLCδ-PH-GFP signal intensity at branch points and on dendrite shafts adjacent to branchpoints. n = 20 branch points from five neurons (100 total). ( E) Co-localization of PLCδ-PH-GFP and coracle. Maximum intensity projections of anti-GFP and anti-coracle immunostaining of larva expressing OSH2-GFP in the epidermis. ( F) Sheath localization of the septate junction protein Neurexin-IV. Maximum intensity projections of Neurexin-IV-GFP and ppkCD4-tdTomato are shown. Arrows mark sheaths labeled by Neurexin-IV-GFP. |